GSM4653910: Ferrous iron treatment_rep1; Streptococcus suis; RNA-Seq - SRA

SRX8664943: GSM4653910: Ferrous iron treatment_rep1; Streptococcus suis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 5.9M spots, 1.8G bases, 531.6Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptomic analysis of Streptococcus suis in response to ferrous iron and cobalt toxicity

PRJNA643997 • SRP269943 • All experiments • All runs

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We explored the transcriptome change of Streptococcus suis in the presence of ferrous iron and cobalt by RNA sequencing. The data revealed that expression of a lot of genes was altered to respond to ferrous iron and cobalt toxicity. Moreover, most of the genes differentially expressed in response to cobalt are the same as those in response to ferrous iron. Overall design: The transcription profiles of Streptococcus suis following treatment with ferrous iron and cobalt were compared with that treatment with water. Each condition contains three biological replications.

Sample: Ferrous iron treatment_rep1

SAMN15438799 • SRS6945864 • All experiments • All runs

Organism: Streptococcus suis

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was extracted using an Eastep Super Total RNA Isolation Kit (Promega, Shanghai, China), according to the manufacturer's instructions. RNA integrity and concentration were evaluated by gel electrophoresis and spectrophotometric analysis, respectively. A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.

Experiment attributes:

GEO Accession: GSM4653910

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 5.9M spots, 1.8G bases, 531.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR12143889	5,880,133	1.8G	531.6Mb	2020-07-05

ID:: 11272961

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